|
KMID : 0613820110210010036
|
|
Journal of Life Science
2011 Volume.21 No. 1 p.36 ~ p.43
|
|
|
Glycated Serum Albumin Induces Interleukin-6 Expression in Vascular Smooth Muscle Cells
|
|
Baek Seung-Il
Rhim Byung-Yong
Kim Koan-Hoi
|
|
|
Abstract
|
|
|
|
Diabetes mellitus is associated with vascular complications. Diabetic patients exhibit high levels of glycated adducts in serum compared to non-diabetic individuals. The aim of this study was to investigate whether extracellular glycated albumin (GA) predisposes vascular smooth muscle cells (VSMCs) to pro-inflammatory phenotype. Exposure of rat aortic smooth muscle cells (AoSMCs) to GA not only enhanced interleukin-6 (IL-6) release but also activated promoter activity of the IL-6 gene. GA-induced IL-6 promoter activation was suppressed by dominant-negative forms of Toll-like receptor (TLR)-4 and myeloid differentiation factor 88 (MyD88), but not by dominant-negative-forms of TLR-2 and TIR-domain-containing adapter-inducing interferon-β (TRIF). Extracellular signal-regulated kinase (ERK) inhibition and diphenyleneiodium (DPI) also attenuated IL-6 induction by GA. Mutation at the nuclear factor-κB (NF- κB)-binding site in the IL-6 promoter region suppressed promoter activation in response to GA. The present study proposes that GA would contribute to inflammatory reaction in the stressed vasculature by inducing IL-6 in VSMCs, and that TLR-4, EKR, and NF-κB play active roles in the process.
|
|
|
KEYWORD
|
|
|
Interleukin-6 (IL-6), glycated serum albumin, vascular smooth muscle cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|